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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been observed in many tumors, which leads to chromosome instability, and thus may possibly lead to tumorigenesis. This protein was discovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in endorsing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been claimed but specific nucleotide sequence info is not really nevertheless accessible.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally incorporates the epitope identified with the antibody.
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This guideline outlines even further attainable optimization of mobile and immunoassay parameters to ensure the absolute best effects are...
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This guideline outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody by yourself, you can see which staining is particular.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add towards the temporal coordination of each mitotic function. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is thr77 login degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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